1. Prepare your template

Please prepare your template using our checklist:

  • Ensure that any kits you use to prepare samples specify that DNA sequencing is a downstream application.
  • Submit DNA templates in either sterile water or 10mM Tris pH 8.0.
  • Supply 10 uL of DNA per reaction. Only 1 uL of DNA is used per reaction but providing additional volume allows for re-runs if necessary. If sample volume is a concern, please discuss with the Lab Coordinator.
  • Review the following table for recommended quantity measurements. Final concentration will be in units of ng/uL or ug/uL depending on DNA template.

 

Template DNAs

Quantity per reaction
  • 100-200 bp
 1-3 ng
  • 200-500 bp
 3-10 ng
  • 500-1000 bp
 5-10 ng
  • 1000-2000 bp
 10-40 ng
  • more than 2000 bp
 25-50 ng
Single-stranded DNA 25-50 ng
Double-stranded DNA 150-300 ng
Cosmid, BAC 0.5-1.0 µg
Bacterial genomic DNA 0.5-1.0 µg

 

Fragment Analysis

Fragment analysis plates should be fully prepared by the client and will be run as is.