1. Prepare your template
Please prepare your template using our checklist:
- Be sure that the kit you are using specifies automated DNA sequencing is a downstream application for DNA processed in that manner.
- Submit your DNA templates in either sterile water or 10mM Tris pH 8.0.
- Do not submit DNA in TE or containing gel tracking dye.
- Review the following table for recommended concentration measurements:
|PCR fragments||5-10ng/ul||up to 100ng|
|Bacterial genomic DNA||500ng/ul||2-3ug|