1. Prepare your template

Please prepare your template using our checklist:

  • Be sure that the kit you are using specifies automated DNA sequencing is a downstream application for DNA processed in that manner.
  • Submit your DNA templates in either sterile water or 10mM Tris pH 8.0.
  • Do not submit DNA in TE or containing gel tracking dye.
  • Review the following table for recommended concentration measurements:
Template DNAs Concentration Quantity
per reaction
Single-stranded DNA 10ng/ul 50-100ng
Plasmid DNA 100ng/ul 200-500ng
PCR fragments 5-10ng/ul up to 100ng
Large DNA 200ng/ul 1ug
Bacterial genomic DNA 500ng/ul 2-3ug