Sample Preparation
Normalization
Calculates and dilutes DNA samples to a concentration suitable for Sanger sequencing or another desired application.
Purification- ExoSAP
Enzymatic purification method provides 100% recovery of amplicons regardless of fragment size.
Purification- QIAquick
Spin column purification method provides up to 95% recovery of amplicons between 100bp – 10kb.
Quantification – NanoDrop
Absorbance-based assay can provide purity ratios such as A260/A280 and identify contaminants.
Quantification – Qubit
Fluorescence based assay is nucleic acid specific and can accurately measure both dilute samples and samples with contaminates.