Services Available
Survey design consultation
Sample collection
Sample filtration
DNA extraction
Inhibition testing and clean-up
Starting copy number calculation
Results summary with quality controls
Survey interpretation and analysis consulting
Training
Staff can conduct individual or group training on all aspects of eDNA either on site (UMaine) or offsite.
NGS library prep
Sample testing
We can test for your target species using qPCR assays or metabarcoding. In the near future we will offer ddPCR. Our equipment is also available for non-eDNA related testing. Please see the list of available assays on this webpage.
In-house qPCR assays:
Alewife/Blueback herring (Alosa pseudoharengus, alosa aestivalis)
American shad (Alosa sapidissima)
Arctic charr (Salvelinus alpinus)
Atlantic herring (Clupea harengus)
Atlantic mackerel (Scomber scombrus)
Atlantic salmon (Salmo salar)
Atlantic sturgeon (Acipensir oxyrhynchus oxyrhynchus)
Black crappie (Pomoxis nigromaculatus)
Brook trout (Salvelinus fontinalis)
Chain pickerel (Esox niger)
Chloroplast
Lake trout (Salvelinus namaycush)
Largemouth bass (Micropterus salmoides)
Mudpuppy (Necturus maculosus)
Muskellunge (Esox masquinongy)
Northern pike (Esox lucius)
Pollock (Pollachius virens)
Pumpkinseed sunfish (Lepomis gibbosus)
Rainbow smelt (Osmerus mordax)
Redbreast sunfish (Lepomis auritus)
Sea lamprey (Petromyzon marinus)
Shortnose sturgeon (Acipenser brevirostrum)
Smallmouth bass (Micropterus dolomieu)
Striped bass (Morone saxatilis)
White perch (Morone americana)
In addition to in-house assays, we can also test for any species with publicly published assays, contact us to find out if your target is available.
We currently test using qPCR, and will soon offer quantitative eDNA testing via ddPCR as well as Next-Gen sequencing via Illumina Miseq. Please contact us with any questions.