Zeiss LSM 980 – Airyscan 2

The inverted laser scanning confocal microscope is ideal for diverse range of applications for confocal and super resolution imaging including multi-color experiments, live sample imaging, protein colocalization, tiling of large samples, Z-stack 3D/4D imaging, and weak label detection. The microscope is equipped with environmental control (temperature, humidity and CO 2 ) and can image live BSL-2 samples. The
motorized XY stage can hold samples in slides, 35 mm dishes, chambered slides, and well-plates.


Airyscan 2

  • Super resolution with high light efficiency
  • Multiplex mode collects image up to 10 times faster

Lasers – all diode lasers

  • 405 nm
  • 445 nm
  • 488 nm
  • 561 nm
  • 639 nm
Confocal slice in a muscle fiber in a fish fixed at 3 dpf, when myofibers have become contractile. This imaging offers a high-resolution view of sarcomeres, which are the fundamental contractile unit of muscle; sarcomeric structure is disrupted by mutants studied in the Talbot lab. The white membrane label is six1b:lyn-GFP, green shows the myosin heavy chain antibody A4.1025, red is the F-Actin stain phalloidin, blue is the muscle nucleus marker Rbfox1l.

Objectives

  • EC Plan-Neofluar 10×/0.3 air
  • Objective PApo 20×/0.8 high resolution air
  • Long working distance 40×/1.1 water
  • Objective C PApo 63×/1.4 oil

Detectors

  • 2 photomultiplier tubes (PMTs)
  • High sensitivity GaAsP detector
  • Airyscan detector with 4Y and 8Y fast mode
  • Transmitted light T-PMT detector

Software: Zen 3.8 for microscope control, image acquisition and processing.

Acknowledging the MIAC facility: the NIH requires that all publications containing data
collected on the microscope, including press releases, acknowledge NIH grant support.
Please use the following format: Research reported in this [publication, release] was
supported by Award number 1P20GM144265 from the National Institutes of
Health.

Please contact MIAC facility manager Zhengxin (Zhengxin.ma@maine.edu) for more
information. We look forward to working with you!

Sites of Endo cytosis Indicated by Cortical Actin Patches. Yeast (S. cerevisiae) expressing a mutant RGS (sst2S539A) were treated with saturating pheromone followed by staining of the cell wall and actin (Calcofluor White in blue and phalloi din-CF488 in green).