Media
Tnag
0.8 g (Tryptone)
0.4 g (n-acetyl glucosamine)
5 g (agar)
500 mL (water)
Murray & Lovett code = M & L
0.6 g (glucose)
0.3 g (n-acetyl glucosamine)
0.25 g (asparagine)
1.0 g (tryptone)
7.0 g (agar)
50 mL (Machlis’ soln)
10X Machlis’ solution
1 g ( NH4NO3 [5X10-3 M] )
0.18 g ( CaCl2 [5X10-4 M] )
0.15 g ( MgSO4 [5 X 10-3 M] )
3.35 g ( Na2HPO4 [ 5 X 10-3 M] )
1.7 g (KH2PO4 [ 5 X 10-3 M] )
250 mL (Distilled Water)
YpSs/2 agar code = Y/2
10.1 g (Emerson’s YpSs agar)
500 mL (Distilled Water)
* add 1 g agar for slants
PVLG
8.33 g (Polyvinyl alcohol)
50 mL (Lactic acid)
5 mL (glycerine)
50 mL (Distilled water)
TYG
1.25 g (tryptone)
0.625 g (yeast extract)
1.5 g (dextrose)
500 mL (distilled water)
T/2 broth
2.5 g (tryptone)
500 mL (distilled water)
PYG
0.6 (peptone)
0.6 g (yeast extract)
3 g (glucose)
3 g (agar)
500 mL (distilled water)
YsSs/2 broth
1 g (bacto yeast extract)
3.75 g (soluble starch)
0.25 g (dipotassium phosphate)
0.1 g (magnesium sulfate)
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Dilute Soluble Starch = dSS or d
0.1 g (peptonized milk)
0.1 g (tryptone)
2 g (soluble starch)
500 mL (distilled water)
5 g (agar) *for plates
6 g (agar) *for tubes
Cellobiose-Tryptone agar code = CT
0.2 g (tryptone)
2 g (cellobiose)
5 g (agar)
500 mL (distilled water)
Harpochytrium broth code = H
10 g (tryptone)
3.2 g (glucose)
1000 mL (distilled water)
Koch’s K-1 agar
0.3 g (peptone)
0.2 g (yeast extract)
0.6 g (glucose)
5 g (agar)
500 mL (distilled water)
Lima Bean medium
Frozen lima beans 1 package (boil 1/2 hour in
1000 mL distilled water, filter through cheese cloth
and replenish water to 1 liter); 10 g agar
for agar medium and no agar for broth
PmTG code = P
0.5 g (peptonized milk)
0.5 g (tryptone)
2.5 g (glucose)
5 g (agar) * for plates
6 g (agar) * for tubes)
500 mL (distilled water)
xylan agar code = x
0.5 g (peptonized milk)
0.5 g (tryptone)
2.5 g (xylan)
500 mL (distilled water)
5 g (agar) * for plates
6 g (agar) * for tubes
mPmTG code = m
0.2 g (peptonized milk)
0.2 g (tryptone)
1.0 g (glucose)
500 mL (distilled water)
5 g (agar) *for plates
6 g (agar) *for tubes
mTGh
4 g (tryptone)
1 g (gelatin hydrolysate)
5 g or 6 g (agar)
500 mL (distilled water)
Antibiotics
Penicillin-Streptomycin code is +.
Add antibiotics after autoclaving. Penicillin200 mg/L
Streptomycin sulfate200 – 500 mg/L
Autoclave
121 degrees Celsius, (15 p.s.i.)
for 30 minutes
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