UMaine CORE Genetics

About Us

The University of Maine CORE Genetics Facilities provide comprehensive molecular biology and genomics services that support research, environmental monitoring, education, and innovation across Maine and beyond. Serving academic investigators, government agencies, industry partners, non-profit organizations, and community groups, the facilities deliver expert technical services, state-of-the-art instrumentation, and collaborative scientific support for projects ranging from routine DNA analysis to advanced environmental genomics.

The CORE DNA Sequencing Facility provides high-quality Sanger DNA sequencing and fragment analysis services for researchers across the University of Maine System and external organizations. Equipped with high-throughput capillary sequencing instrumentation, the facility supports a broad range of applications including DNA sequence verification, mutation detection, microsatellite analysis, SNP genotyping, and fragment analysis. Flexible service options accommodate both full-service sequencing and client-prepared samples, providing rapid, reliable, and cost-effective molecular analysis for research, education, and diagnostic applications.

Person taking samples

Environmental DNA (or eDNA) is simply genetic material that is present and detectable in environmental sources, such as water, soil or air.  It comes from shed cells or decomposition products of larger organisms, like fish or plants, or can be from whole microorganisms like plankton and bacteria.  Because DNA is an almost universal code for life, eDNA detection and quantification can be used to understand what organisms currently or previously occupied a given environment, providing a power tool to monitor, study, and manage biodiversity.  The University of Maine CORE Environmental DNA Laboratory was established in 2019 through an investment of the Office of the Vice President of Research and Dean of the Graduate School to establish the Coordinated Operating Research Entities (CORE) program and contributions to major equipment from the NSF EPSCoR RII Track 1 Program (Grant # 11A-1849227). We provide eDNA services for academic, government, non-governmental, industry and citizen group organizations.

miseq machine picture
Laura Jackson

Laura Jackson, Ph.d.

Bioinformatician

laura.jackson@maine.edu

(207) 581-1451

Megan Franz

Megan Franz

Lab Coordinator

megan.franz@maine.edu

(207) 581-2795

Services and Pricing

We offer high or low throughput DNA extraction services on a large number of sample types. Our extraction products are high-quality and are perfectly suited for your downstream applications, including PCR or next-generation sequencing. Please contact the laboratory directly at um.genetics@maine.edu for an estimate of turnaround time, or if you are planning on submitting a large project.

ServiceDescriptionUMS Per Sample CostAcademic / Non-Profit Per Sample CostExternal Per Sample Cost
DNA ExtractionExtraction of DNA from samples. Kits charged separately.$8.50 / Sample$9.75 / Sample$10.50 / Sample
Gel ElectrophoresisSeparation and visualization of DNA fragments on an agarose gel.
Confirms PCR amplification and fragment size.
$70.00 / Gel$80.00 / Gel$87.50 / Gel
Purification: ExoSAPEnzymatic Purification method provides 100% recovery of amplicons regardless of fragment size.
$5.00 / Sample

$5.75 / Sample

$6.00 / Sample
Purification: QIAquickSpin column purification method provides up to 95% recovery of amplicons between 100bp – 10kb.
$6.00 / Sample

$7.00 / Sample

$7.50 / Sample
Quantification: QubitTM Fluorometer MeasurementDNA quantification.
$5.00 / Sample

$5.75 / Sample

$6.25 / Sample
PCRDNA Amplification.
Price per 96-well plate.

$200.00 / Plate

$230.00 / Plate

$250.00 / Plate

BioRad ddPCR
Absolute quantification of DNA.
$40.00 block

$44.00 block

$50.00 block

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

We offer environmental DNA sample processing services on a large number of sample types and project sizes. To submit a project inquiry, get an estimated turnaround time, or request a quote, please complete the eDNA Project Request form within the CORE Genetics Facility iLab page.

ServiceDescriptionUMS Per Sample CostAcademic / Non-Profit Per Sample CostExternal Per Sample Cost
eDNA Sample FilteringVacuum filtration of water samples for eDNA collection$10.50 / Liter Sample$12.00 / Liter Sample$13.00 / Liter Sample

eDNA Filter Extraction
Extraction of eDNA from water sample filters.
Specialized kits available per request and charged as add-on.

$10.50 / Sample

$12.00 / Sample

$13.00 / Sample

Sample Clean-Up
Purification of extracted eDNA to increase PCR efficiency using Zymo PCR Inhibitor Removal Kit.
$9.00 / Sample

$10.50 / Sample

$12.00 Sample
Sample NormalizationNormalization of usable libraries across samples prior to sequencing.
Charged by plate of 96.
$35.00 / Plate$40.25 / Plate$45.00 / Plate

Targeted eDNA Assay Single Probe qPCR (4 Replicates)
Batch of 4 qPCR replicates using a single Taqman MGB-NFQ probe assay.
$26.75 / Sample

$30.00 / Sample

$35.00 / Sample
Targeted eDNA Assay Double Probe qPCR (4 Replicates)Batch of 4 qPCR replicates using two Taqman MGB-NFQ probe assays.$26.75 / Sample$30.00 / Sample$35.00 / Sample

Custom qPCR Assay
Custom qPCR assay development services are available. We can develop new assays for your particular application or adapt those from the scientific literature.
Contact the lab for more information on pricing

Contact the lab for more information on pricing

Contact the lab for more information on pricing

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

ServiceDescriptionUMS CostAcademic / Non-Profit Per Sample CostExternal Per Sample Cost
Sanger Sequencing – Cycle SequencingCycle sequencing, ETOH/EDTA clean up, and electrophoresis of DNA samples.
The lab uses the Big Dye brand of reagents.

$4.50 / reaction

$5.00 / reaction

$5.50 / reaction

Electrophoresis
Provides capillary electrophoresis of a client’s prepared plate for Sanger Sequencing.
$1.75 / reaction

$2.00 / reaction

$2.15 / reaction

Fragment Analysis
Electrophoresis and sizing of DNA samples using ABI Genescan Analysis Software.
$1.75 / fragment

$2.00 / fragment

$2.15 / fragment

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

Costs do not include any bioinformatics analyses of generated data. Clients will be provided with the raw run data and basecall files to use in their own downstream processes. See Technical Consulting and Bioinformatics Services for more information.

ServiceDescriptionUMS Per Run CostAcademic / Non-Profit Per Run CostExternal Per Run Cost
Illumina IndexingIndexing using Illumina UDI for multiplexing up to 384 samples.$375.00 / run$410.00 / run$435.00 / run

MiSeq Sequencing run

Next-generation sequencing (Reagent kit billed separately)

$400.00 / Run

$435.00 / Run

$480.00 / Run
MiSeq Reagent: PhiX Control v3 (Illumina)Item / Catalog #: FC-110-3001
Price is charged at cost on the day ordered.
$20.24$20.75$20.75
Miseq Kits: v2 300 Cycle Nano Kit (Illumina)Item/Catalog #: MS-103-1001
Price is charged at cost on the day ordered.
Price is charged at cost on the day ordered.Price is charged at cost on the day ordered.Price is charged at cost on the day ordered.
MiSeq Kits: v3 600 Cycle (Illumina)Item/Catalog #: MS-102-3003Price is charged at cost on the day ordered.Price is charged at cost on the day ordered.Price is charged at cost on the day ordered.
MiSeq Kits: v3 150 Cycle (Illumina)Item / Catalog #: MS-102-3001Price is charged at cost on the day ordered.Price is charged at cost on the day ordered.Price is charged at cost on the day ordered.

Current standardized primers:

Beyond the 12S/16S/18S/ITS/COI amplicons offered “in-stock”, essentially any PCR amplicon (max. size ~450-500 bp for MiSeq) can be adapted to our systems. Please consults us for primer design/protocol details.

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

ServiceDescriptionUMS CostAcademic / Non-Profit Per Sample CostExternal Per Sample Cost
Oxford Nanopore IndexingIndexing for Nanopore sequencing for multiplexing 96 samples per native barcode.$315.00$330.00$350.00
Oxford Nanopore MinION Amplicon sequencingPrice includes the library preparation and sequencing for 6 native barcodes (can barcode individual, mixed samples or dual-indexed libraries for metabarcoding) on a MinION flowcell using the Nanopore Mk1D device. Typical output: 30–50 Gb of data in a single run. 
Flowcell charged separately.
HPC charged separately as applicable
$350.00$415.00$450.00

Oxford Nanopore Small to Medium Sized Genome Sequencing
Price includes the library preparation and sequencing of small to medium-sized genomes on a MinION flow cell using the Mk1D sequencing device. Suitable for bacterial (<10 Mb), fungal/yeast (10-100Mb), and small eukaryotic genomes.
Flowcells charged separately
HPC charged separately as applicable
$385.00$450.00$485.00

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

The CORE Genetics facilities offers bioinformatics training, consulting, data analysis, and custom pipeline development services. We have a number of skilled laboratory technicians and PhD-level staff who can assist you with your project needs. Technician time is billed separately when it is needed outside of the other listed services. This can include sample handling and preparation before extraction procedures, moving samples between vessel types, or consultation on the interpretation of generated data.

ServiceDescriptionUMS Per Hour RateAcademic / Non-Profit Per Hour RateExternal Per Hour Rate

Laboratory technician time
Rate applies to activities performed by the Genetics Lab Coordinator which are not included in the established service offerings. Examples include training, sample processing, and troubleshooting.
$57.50 / hr

$66.00 / hr

$75.00 / hr
Bioinformatics consulting timeRate applies to activities performed by the CORE bioinformatics expert which are not included in the established service offerings. Examples include training, pipeline development, and computational support.
$90.00 / hr

$100.00 / hr

$115.00 / hr
Standard Metabarcoding analysis (12S, 16S, 18S, COI)Contact the lab for more information on pricingContact the lab for more information on pricingContact the lab for more information on pricing
Bioinformatics custom servicesCreation of custom bioinformatics pipelines or additional analysis needs that are not standard services. Extensive code generation may be required and the availability of this service will depend on staff availability. Please contact the lab at um.genetics@maine.edu if you are planning on using this service.Contact the lab for more information on pricingContact the lab for more information on pricingContact the lab for more information on pricing

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

We currently have qPCR and RT-qPCR assays available for a number of vertebrate and invertebrate species. Custom qPCR assay development services are available. We can develop new assays for your particular application or adapt those from the scientific literature. Contact the lab directly at um.genetics@maine.edu to learn more about our species assay availability.

Assay CategoryCommon NameScientific Name
SalmonidsAtlantic salmonSalmo salar
Brook troutSalvelinus fontinalis
Arctic charrSalvelinus alpinus
Brown troutSalmo trutta
Lake troutSalvelinus namaycush
Chinook salmonOncorhynchus tshawytscha
Chum salmonOncorhynchus keta
Diadromous / Migratory FishAlewifeAlosa pseudoharengus
American eelAnguilla rostrata
Rainbow smeltOsmerus mordax
Atlantic sturgeonAcipenser oxyrinchus
Shortnose sturgeonAcipenser brevirostrum
Marine & Estuarine FishAtlantic herringClupea harengus
Atlantic mackerelScomber scombrus
PollockPollachius virens
Atlantic tomcodMicrogadus tomcod
Striped bassMorone saxatilis
White perchMorone americana
Freshwater FishLargemouth bassMicropterus salmoides
Smallmouth bassMicropterus dolomieu
Black crappiePomoxis nigromaculatus
Redbreast sunfishLepomis auritus
Chain pickerelEsox niger
Bridle shinerNotropis bifrenatus
Freshwater MusselsBrook floater musselAlasmidonta varicosa
Yellow lampmusselLampsilis cariosa
Tidewater mucketLeptodea ochracea
CephalopodsLongfin inshore squidDoryteuthis pealeii
Northern shortfin squidIllex illecebrosus
Invasive / Nuisance SpeciesZebra musselDreissena polymorpha
AmphibiansMudpuppyNecturus maculosus
ReptilesSpotted turtleClemmys guttata
Blanding’s turtleEmydoidea blandingii
Marine MammalsGray sealHalichoerus grypus
Harbor sealPhoca vitulina
InsectsNorthern house mosquitoCulex pipiens
Eastern swamp mosquitoCulex restuans
Aquatic PlantsHydrilla / WaterthymeHydrilla verticillata
Northern watermilfoilMyriophyllum sibiricum

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project.

To schedule self-service activities, please reserve in the iLab calendar. Note: training for equipment and/or safety lab practices are required in order to use self-service spaces and equipment.

ServiceDescriptionUMS Internal Rates

Agilent 4150 TapeStation
Price is per hour with a 15 min. minimum reservation.
The Agilent Tape Station performs automated electrophoresis of DNA or RNA samples and generates
sizing, quantification, and molarity of DNA fragments.

$10.50 / hr

Agilent Cytation 5 Plate Reader
Price is per hour with a 15 min. minimum reservation.
Agilent Cytation Biotek Multi-Mode Plate Reader.
Cytation 5 combines automated microscopy and conventional microplate detection. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast. The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection.
The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 software provides complete control over all imaging and data capture, plus powerful image and data analysis.

$8.40 / hr
Filtering Lab SpaceVacuum filtration of water samples for eDNA collection.$6.50 / liter sample
DNA Extraction Lab SpaceDNA extraction from sample. Specialized kits available per request and charged as add-on.$6.75 / sample
Filter Extraction Lab SpaceVacuum filtration of water samples for eDNA collection. Specialized kits available per request and charged as add-on.$7.00 / sample
Sample Clean-up Lab SpacePurification of extracted eDNA to increase PCR efficiency using Zymo PCR Inhibitor Removal Kit.$7.25 / sample
PCRDNA amplification performed by client. Plate of 48.$165.00 / plate
Targeted eDNA Single Probe qPCR AssayBatch of 4 qPCR replicates using a single Taqman MGB-NFQ probe assay. Work performed by client.$18.60 / assay
Targeted eDNA Double Probe qPCR AssayBatch of 4 qPCR replicates using two Taqman MGB-NFQ probe assays. Work performed by client.$19.25 / assay
ddPCRAbsolute quantification of DNA. Work performed by client$35.00 / run
Qubit QuantificationDNA quantification performed by client. Fluorescence based assay is nucleic acid specific and can accurately measure both dilute samples and samples with contaminates.$3.75 / sample

Pre-payments are not allowed under any circumstances. All payments must be for services rendered. Services purchased with sponsored funds must be directly allocable to the funding project. Prices updated as of 10/01/2026.

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