News

New paper on the effects of feedback phosphorylation of the RGS, Sst2.

We have recently published a paper in Life Science Alliance, entitled “Phosphorylation of RGS regulates MAP kinase localization and promotes completion of cytokinesis.”  This work was spearheaded by master’s student Will Simke and PhD student Cory Johnson, co-first authors on the final work. When yeast respond to mating pheromone, they must complete their current round […]

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Biomed Data Science Class

Dr. Kelley is leading a python class for graduate students in the biomedical sciences at UMaine.  We will be using DataCamp to explore programming, data analysis and graphing in Python.  If you are interested in joining in, please send Dr. Kelley an email.   The class is being done through DataCamp for the Classroom, an […]

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Kelley Lab develops image analysis of Neuromuscular Junction

In collaboration with the Henry Lab at UMaine, the Kelley Lab has developed a method to quantify innervation of the Neuromuscular Junctions in zebra fish to assist in the Henry lab’s work on dystroglycanopathies.  This work has now been published in Skeletal Muscle: NAD+ improves neuromuscular development in a zebrafish model of FKRP-associated dystroglycanopathy.  Bailey […]

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William Simke completes his Master’s Degree!

Will Simke successfully defended his thesis entitled “Dynamic Regulation of G-Protein Signaling” and has graduated with his Master’s degree.  Will has enrolled at UNC Chapel Hill to pursue his PhD in Chemistry.

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Kelley Lab Publishes paper on Quantification of Nuclear Fluorescence

This paper describes the considerations and approaches necessary for quantification of nuclear and cytoplasmic distribution of molecules imaged by fluorescence microscopy. It covers the use of ImageJ for performing the quantitation as well as simple approaches to intensity based segmentation of images for higher throughput measurements. Fluorescence-based quantification of nucleocytoplasmic transport. Kelley JB, Paschal BM. Methods. […]

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Kelley Lab Publishes Paper on Stress Effects on RNA Processing Enzyme Localization

  In Collaboration with the Reines Lab at Emory University, the Kelley Lab has published a new paper in PLoS ONE. Nab3’s localization to a nuclear granule in response to nutrient deprivation is determined by its essential prion-like domain. Loya TJ, O’Rourke TW, Simke WC, Kelley JB, Reines D. PLoS One. 2018 Dec 17;13(12):e0209195. doi: 10.1371/journal.pone.0209195. […]

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Kelley Lab receives Grant from NIH to Study G-alpha Signaling

The Kelley Lab, in collaboration with Andre Khalil and the CompuMAINE lab, has received a 3 year, $424,438 grant from the NIH to investigate the role of G-alpha signaling in regulating gradient tracking.  This project involves a combination of yeast genetics, live cell microscopy in microfluidic devices, computational image analysis, and computational modeling.

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