Kelley Lab at UMaine

We have recently published a paper in Life Science Alliance, entitled “Phosphorylation of RGS regulates MAP kinase localization and promotes completion of cytokinesis.”  This work was spearheaded by master’s student Will Simke and PhD student Cory Johnson, co-first authors on the final work. When yeast respond to mating pheromone, they must complete their current round […]

In collaboration with the Henry Lab at UMaine, the Kelley Lab has developed a method to quantify innervation of the Neuromuscular Junctions in zebra fish to assist in the Henry lab’s work on dystroglycanopathies.  This work has now been published in Skeletal Muscle: NAD+ improves neuromuscular development in a zebrafish model of FKRP-associated dystroglycanopathy.  Bailey […]

This paper describes the considerations and approaches necessary for quantification of nuclear and cytoplasmic distribution of molecules imaged by fluorescence microscopy. It covers the use of ImageJ for performing the quantitation as well as simple approaches to intensity based segmentation of images for higher throughput measurements. Fluorescence-based quantification of nucleocytoplasmic transport. Kelley JB, Paschal BM. Methods. […]

In collaboration with the Paschal Lab at the University of Virginia, the Kelley Lab has published a new paper in Aging Cell.  Kelley Lab members William Simke and Isaac Johnson generated yeast missing a key histone methyltransferase and examined the ability of the cell to properly localize Ran.  Ran is the main regulator of nucleocytoplasmic […]

  In Collaboration with the Reines Lab at Emory University, the Kelley Lab has published a new paper in PLoS ONE. Nab3’s localization to a nuclear granule in response to nutrient deprivation is determined by its essential prion-like domain. Loya TJ, O’Rourke TW, Simke WC, Kelley JB, Reines D. PLoS One. 2018 Dec 17;13(12):e0209195. doi: 10.1371/journal.pone.0209195. […]