Zeiss LSM980 – Airyscan 2
The inverted laser scanning confocal microscope is ideal for diverse range of applications for confocal and super resolution imaging including multi-color experiments, live sample imaging, protein colocalization, tiling of large samples, Z-stack 3D/4D imaging, and weak label detection. The microscope is equipped with environmental control (temperature, humidity and CO2) and can image live BSL-2 samples. The motorized XY stage can hold samples in slides, 35 mm dishes, chambered slides, and well-plates.
Airyscan 2
• Super resolution with high light efficiency
• Multiplex mode collects image up to 10 times faster
Lasers – all diode lasers
• 405 nm
• 445 nm
• 488 nm
• 561 nm
• 639 nm
Objectives
• EC Plan-Neofluar 10×/0.3 air
• Objective PApo 20×/0.8 high resolution air
• Long working distance 40×/1.1 water
• Objective C PApo 63×/1.4 oil
Detectors
• 2 photomultiplier tubes (PMTs)
• High sensitivity GaAsP detector
• Airyscan detector with 4Y and 8Y fast mode
• Transmitted light T-PMT detector
Software: Zen 3.8 for microscope control, image acquisition and processing
Acknowledging miac:
the NIH requires that all publications containing data collected on the microscope, including press releases, acknowledge NIH grant support. Please use the following format:
The authors would like to acknowledge the Microscopy and Image Analysis Core (MIAC) facility (RRID: RRID:SCR_025784) for use of Zeiss LSM980 Confocal Microscope/Imaris software and staff XXX for helpful training/discussion/collecting the data shown in Figure Y. The MIAC facility was supported by Award number 1P20GM144265 from the National Institutes of Health.