Maine Chytrid Laboratory

Tnag

0.8 g (Tryptone)

0.4 g (n-acetyl glucosamine)

5 g (agar)

500 mL (water)

Murray & Lovett code = M & L

0.6 g (glucose)

0.3 g (n-acetyl glucosamine)

0.25 g (asparagine)

1.0 g (tryptone)

7.0 g (agar)

50 mL (Machlis’ soln)

10X Machlis’ solution

1 g ( NH₄NO₃ [5X10^-3 M] )

0.18 g ( CaCl₂ [5X10^-4 M] )

0.15 g ( MgSO₄ [5 X 10^-3 M] )

3.35 g ( Na₂HPO₄ [ 5 X 10^-3 M] )

1.7 g (KH₂PO₄ [ 5 X 10^-3 M] )

250 mL (Distilled Water)

YpSs/2 agar code = Y/2

10.1 g (Emerson’s YpSs agar)

500 mL (Distilled Water)

* add 1 g agar for slants

Autoclave

121 degrees Celsius, (15 p.s.i.) for 30 minutes

Compounds

PVLG

8.33 g (Polyvinyl alcohol)

50 mL (Lactic acid)

5 mL (glycerine)

50 mL (Distilled water)

TYG

1.25 g (tryptone)

0.625 g (yeast extract)

1.5 g (dextrose)

500 mL (distilled water)

T/2 broth

2.5 g (tryptone)

500 mL (distilled water)

PYG

0.6 (peptone)

0.6 g (yeast extract)

3 g (glucose)

3 g (agar)

500 mL (distilled water)

YsSs/2 broth

1 g (bacto yeast extract)

3.75 g (soluble starch)

0.25 g (dipotassium phosphate)

0.1 g (magnesium sulfate)

Lactophenol

20 g (phenol crystals)

20 g / 16 mL (lactic acid)

40 g / 31 mL (glycerol)

20 g / 20 mL (distilled water)

0.05 g – 1.0 g (cotton blue)

Antibiotics

Penicillin-Streptomycin code is +.

Add antibiotics after autoclaving. Penicillin 200 mg/L

Streptomycin sulfate 200 – 500 mg/L

Media

dSS or d = dilute soluble starch

0.1 g (peptonized milk)

0.1 g (tryptone)

2 g (soluble starch)

500 mL (distilled water)

5 g (agar) *for plates

6 g (agar) *for tubes

Cellobiose-Tryptone

agar code = CT

0.2 g (tryptone)

2 g (cellobiose)

5 g (agar)

500 mL (distilled water)

Harpochytrium broth

code = H

10 g (tryptone)

3.2 g (glucose)

1000 mL (distilled water)

Koch’s K-1 agar

0.3 g (peptone)

0.2 g (yeast extract)

0.6 g (glucose)

5 g (agar)

500 mL (distilled water)

Lima Bean – medium

Frozen lima beans 1 package (boil 1/2 hour in

1000 mL distilled water, filter through cheese cloth

and replenish water to 1 liter); 10 g agar

for agar medium and no agar for broth

PmTG

code = P

0.5 g (peptonized milk)

0.5 g (tryptone)

2.5 g (glucose)

5 g (agar) * for plates

6 g (agar) * for tubes)

500 mL (distilled water)

xylan agar

code = x

0.5 g (peptonized milk)

0.5 g (tryptone)

2.5 g (xylan)

500 mL (distilled water)

5 g (agar) * for plates

6 g (agar) * for tubes

mPmTG

code = m

0.2 g (peptonized milk)

0.2 g (tryptone)

1.0 g (glucose)

500 mL (distilled water)

5 g (agar) *for plates

6 g (agar) *for tubes

mTGh

4 g (tryptone)

1 g (gelatin hydrolysate)

5 g or 6 g (agar)

500 mL (distilled water)