Baits and Culture Recipes
Tnag0.8 g (Tryptone) 0.4 g (n-acetyl glucosamine) 5 g (agar) 500 mL (water) Murray & Lovett code = M & L0.6 g (glucose) 0.3 g (n-acetyl glucosamine) 0.25 g (asparagine) 1.0 g (tryptone) 7.0 g (agar) 50 mL (Machlis’ soln) 10X Machlis’ solution1 g ( NH4NO3 [5X10-3 M] ) 0.18 g ( CaCl2 [5X10-4 M] ) 0.15 g ( MgSO4 [5 X 10-3 M] ) 3.35 g ( Na2HPO4 [ 5 X 10-3 M] ) 1.7 g (KH2PO4 [ 5 X 10-3 M] ) 250 mL (Distilled Water) YpSs/2 agar code = Y/210.1 g (Emerson’s YpSs agar) 500 mL (Distilled Water) * add 1 g agar for slants Autoclave121 degrees Celsius, (15 p.s.i.) for 30 minutes Compounds PVLG8.33 g (Polyvinyl alcohol) 50 mL (Lactic acid) 5 mL (glycerine) 50 mL (Distilled water) TYG1.25 g (tryptone) 0.625 g (yeast extract) 1.5 g (dextrose) 500 mL (distilled water) T/2 broth2.5 g (tryptone) 500 mL (distilled water) PYG0.6 (peptone) 0.6 g (yeast extract) 3 g (glucose) 3 g (agar) 500 mL (distilled water) YsSs/2 broth1 g (bacto yeast extract) 3.75 g (soluble starch) 0.25 g (dipotassium phosphate) 0.1 g (magnesium sulfate) Lactophenol20 g (phenol crystals) 20 g / 16 mL (lactic acid) 40 g / 31 mL (glycerol) 20 g / 20 mL (distilled water) 0.05 g – 1.0 g (cotton blue) |
AntibioticsPenicillin-Streptomycin code is +. Add antibiotics after autoclaving. Penicillin 200 mg/L Streptomycin sulfate 200 – 500 mg/L MediadSS or d = dilute soluble starch 0.1 g (peptonized milk) 0.1 g (tryptone) 2 g (soluble starch) 500 mL (distilled water) 5 g (agar) *for plates 6 g (agar) *for tubes Cellobiose-Tryptoneagar code = CT 0.2 g (tryptone) 2 g (cellobiose) 5 g (agar) 500 mL (distilled water) Harpochytrium brothcode = H 10 g (tryptone) 3.2 g (glucose) 1000 mL (distilled water) Koch’s K-1 agar0.3 g (peptone) 0.2 g (yeast extract) 0.6 g (glucose) 5 g (agar) 500 mL (distilled water) Lima Bean – mediumFrozen lima beans 1 package (boil 1/2 hour in 1000 mL distilled water, filter through cheese cloth and replenish water to 1 liter); 10 g agar for agar medium and no agar for broth PmTGcode = P 0.5 g (peptonized milk) 0.5 g (tryptone) 2.5 g (glucose) 5 g (agar) * for plates 6 g (agar) * for tubes) 500 mL (distilled water) xylan agarcode = x 0.5 g (peptonized milk) 0.5 g (tryptone) 2.5 g (xylan) 500 mL (distilled water) 5 g (agar) * for plates 6 g (agar) * for tubes mPmTGcode = m 0.2 g (peptonized milk) 0.2 g (tryptone) 1.0 g (glucose) 500 mL (distilled water) 5 g (agar) *for plates 6 g (agar) *for tubes mTGh4 g (tryptone) 1 g (gelatin hydrolysate) 5 g or 6 g (agar) 500 mL (distilled water) |