Equipment Quantitative PCR (qPCR): Bio-Rad CFX96 Quantitative PCR (qPCR) is a highly sensitive method for detection and relative quantification of eDNA. A qPCR on a Bio-Rad CFX96 real time thermal cycler provides fast turnaround times on samples and relatively simple data to analyze and interpret. qPCR eDNA detection and quantification typically targets highly specific gene