{"id":209,"date":"2019-01-21T11:02:56","date_gmt":"2019-01-21T16:02:56","guid":{"rendered":"https:\/\/umaine.edu\/kelleylab\/?p=209"},"modified":"2019-01-21T11:02:56","modified_gmt":"2019-01-21T16:02:56","slug":"kelley-lab-publishes-paper-on-quantification-of-nuclear-fluorescence","status":"publish","type":"post","link":"https:\/\/umaine.edu\/kelleylab\/2019\/01\/21\/kelley-lab-publishes-paper-on-quantification-of-nuclear-fluorescence\/","title":{"rendered":"Kelley Lab Publishes paper on Quantification of Nuclear Fluorescence"},"content":{"rendered":"
\"\"
Example of the non-intuitive relationship between % Nuclear and N:C ratio.<\/figcaption><\/figure>\n

This paper describes the considerations and approaches necessary for quantification of nuclear and cytoplasmic distribution of molecules imaged by fluorescence microscopy. It covers the use of ImageJ for performing the quantitation as well as simple approaches to intensity based segmentation of images for higher throughput measurements.<\/p>\n

Fluorescence-based quantification of nucleocytoplasmic transport.\u00a0<\/a>Kelley JB, Paschal BM. Methods. 2018 Nov 10. pii: S1046-2023(18)30123-3. doi: 10.1016\/j.ymeth.2018.11.002.\u00a0 PMID: 30419335<\/p>\n","protected":false},"excerpt":{"rendered":"

This paper describes the considerations and approaches necessary for quantification of nuclear and cytoplasmic distribution of molecules imaged by fluorescence microscopy. It covers the use of ImageJ for performing the quantitation as well as simple approaches to intensity based segmentation of images for higher throughput measurements. Fluorescence-based quantification of nucleocytoplasmic transport.\u00a0Kelley JB, Paschal BM. Methods. […]<\/p>\n","protected":false},"author":970,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[6],"tags":[],"class_list":["post-209","post","type-post","status-publish","format-standard","hentry","category-publications"],"yoast_head":"\nKelley Lab Publishes paper on Quantification of Nuclear Fluorescence - Kelley Lab at UMaine - University of Maine<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/umaine.edu\/kelleylab\/2019\/01\/21\/kelley-lab-publishes-paper-on-quantification-of-nuclear-fluorescence\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Kelley Lab Publishes paper on Quantification of Nuclear Fluorescence - Kelley Lab at UMaine - University of Maine\" \/>\n<meta property=\"og:description\" content=\"This paper describes the considerations and approaches necessary for quantification of nuclear and cytoplasmic distribution of molecules imaged by fluorescence microscopy. 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