{"id":2401,"date":"2016-11-02T17:43:44","date_gmt":"2016-11-02T17:43:44","guid":{"rendered":"https:\/\/umaine.edu\/dnaseq\/?page_id=163"},"modified":"2025-06-03T10:46:06","modified_gmt":"2025-06-03T14:46:06","slug":"select-your-primers","status":"publish","type":"page","link":"https:\/\/umaine.edu\/core\/dna-sequencing\/ordering\/select-your-primers\/","title":{"rendered":"2. Select your primers"},"content":{"rendered":"\n<p>When preparing primers:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Submit primers in either sterile water or 10mM Tris pH 8.0.<\/li>\n\n\n\n<li>Primers should be provided at a concentration of 5 uM.<\/li>\n\n\n\n<li>Submit 10 ul of primer per reaction to allow for re-runs if necessary.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>When preparing primers:<\/p>\n","protected":false},"author":2276,"featured_media":0,"parent":2400,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"templates\/page-withsidebar.php","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-2401","page","type-page","status-publish","hentry"],"taxonomy_info":[],"featured_image_src_large":false,"author_info":{"display_name":"jrutherford","author_link":"https:\/\/umaine.edu\/core\/author\/jrutherford\/"},"comment_info":0,"_links":{"self":[{"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/pages\/2401","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/users\/2276"}],"replies":[{"embeddable":true,"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/comments?post=2401"}],"version-history":[{"count":1,"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/pages\/2401\/revisions"}],"predecessor-version":[{"id":2449,"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/pages\/2401\/revisions\/2449"}],"up":[{"embeddable":true,"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/pages\/2400"}],"wp:attachment":[{"href":"https:\/\/umaine.edu\/core\/wp-json\/wp\/v2\/media?parent=2401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}