Baits and Culture Recipes

 

           Media

Tnag

0.8 g (Tryptone)
0.4 g (n-acetyl glucosamine)
5 g (agar)
500 mL (water)

Murray & Lovett code = M & L

0.6 g (glucose)
0.3 g (n-acetyl glucosamine)
0.25 g (asparagine)
1.0 g (tryptone)
7.0 g (agar)
50 mL (Machlis’ soln)

10X Machlis’ solution

1 g ( NH4NO3 [5X10-3 M] )
0.18 g ( CaCl2 [5X10-4 M] )
0.15 g ( MgSO[5 X 10-3 M] )
3.35 g ( Na2HPO4 [ 5 X 10-3 M] )
1.7 g (KH2PO4 [ 5 X 10-3 M] )
250 mL (Distilled Water)

YpSs/2 agar code = Y/2

10.1 g (Emerson’s YpSs agar)
500 mL (Distilled Water)
* add 1 g agar for slants

PVLG

8.33 g (Polyvinyl alcohol)
50 mL (Lactic acid)
5 mL (glycerine)
50 mL (Distilled water)

TYG

1.25 g (tryptone)
0.625 g (yeast extract)
1.5 g (dextrose)
500 mL (distilled water)

T/2 broth

2.5 g (tryptone)
500 mL (distilled water)

PYG

0.6 (peptone)
0.6 g (yeast extract)
3 g (glucose)
3 g (agar)
500 mL (distilled water)

YsSs/2 broth

1 g (bacto yeast extract)
3.75 g (soluble starch)
0.25 g (dipotassium phosphate)
0.1 g (magnesium sulfate)

 

Dilute Soluble Starch  = dSS or d   

0.1 g (peptonized milk)
0.1 g (tryptone)
2 g (soluble starch)
500 mL (distilled water)
5 g (agar) *for plates
6 g (agar) *for tubes

Cellobiose-Tryptone  agar code = CT

0.2 g (tryptone)
2 g (cellobiose)
5 g (agar)
500 mL (distilled water)

Harpochytrium broth code = H

10 g (tryptone)
3.2 g (glucose)
1000 mL (distilled water)

Koch’s K-1 agar

0.3 g (peptone)
0.2 g (yeast extract)
0.6 g (glucose)
5 g (agar)
500 mL (distilled water)

Lima Bean  medium

Frozen lima beans 1 package (boil 1/2 hour in
1000 mL distilled water, filter through cheese cloth
and replenish water to 1 liter); 10 g agar
for agar medium and no agar for broth

PmTG code = P

0.5 g (peptonized milk)
0.5 g (tryptone)
2.5 g (glucose)
5 g (agar) * for plates
6 g (agar) * for tubes)
500 mL (distilled water)

xylan agar code = x

0.5 g (peptonized milk)
0.5 g (tryptone)
2.5 g (xylan)
500 mL (distilled water)
5 g (agar) * for plates
6 g (agar) * for tubes

mPmTG  code = m

0.2 g (peptonized milk)
0.2 g (tryptone)
1.0 g (glucose)
500 mL (distilled water)
5 g (agar) *for plates
6 g (agar) *for tubes

mTGh

4 g (tryptone)
1 g (gelatin hydrolysate)
5 g or 6 g (agar)
500 mL (distilled water)

Antibiotics

Penicillin-Streptomycin code is +.
Add antibiotics after autoclaving. Penicillin200 mg/L
Streptomycin sulfate200 – 500 mg/L

Autoclave

121 degrees Celsius, (15 p.s.i.)
for 30 minutes